The DV2 titer was affected by manipulating autophagy activity in DV2-infected mice brain. Six-day-old ICR suckling mice were pre-treated with 3-MA (80μg/g), rapamycin (0.15μg/g), or PBS by intracranial inoculation, followed by DV2 (2.5 x 105 pfu/mouse) inoculation 2 h later. Plaque assay was conducted to measure the viral titer in DV2-infected mice brain tissue at day 3 and day 5 p.i. in (A) together with 3-MA (DV2+3MA) and at day 5 p.i. in (B) together with rapamycin (DV2+Rapa). PBS-treated group is shown as DV2.