Figure 3

Detection of endogenous ROCKI phosphorylation at Ser1333 residue. (A) HEK293T cells expressing WT or S1333A mutant of flag-ROCKI were harvested for Western blot analysis with anti-pS1333 ROCKI and anti-ROCKI antibodies. (B) Cells were transfected with the expression constructs of GFP, GFP-RhoAV14, GFP-RhoAV14E40L, or GFP-RhoAN19 and harvested for Western blotting with antibodies as indicated. *, non-specific signal.