Figure 4

Effects of WEL on iNOS and COX-2 protein expression in LPS-induced cells. (A) Cells were pre-incubated with WEL (0, 0.1, 1, or 10 μ M) for 12 h, and then incubated with LPS (1 μg/ml) for 20 h. The total protein lysate was subjected to Western blotting. (B) The ratio of immunointensity between the iNOS, COX-2 and the GAPDH was calculated. A representative blot of each experiment is shown with the densitometric analysis corresponding to the mean ± SEM. of three independent experiments. n = 6 per experiment. ^*p < 0.05 vs. LPS group and ^**p < 0.01 vs. LPS group.^#p < 0. 05 vs. none LPS control group. ^##p < 0. 01 vs. none LPS control group.