Figure 2
Effects on the levels of β-catenin, N-cadherin, and Cx 43 in H9c2 cells. (a) Western blotting (top) with quantitative analyses (bottom) of N-Cadherin, β-catenin, and β-actin levels in the H9c2 cells of controls with (lane 2) or without (lane 1) 20 μM EGCg pre-treatment for 30 min or 400 μM H2O2-induced oxidative stress for 30 min with (lane 4) or without (lane 3) 20 μM EGCg pre-treatment. (b) Top: Cx43 levels on western blots using mouse monoclonal anti-rat Cx43 antibody (sc-13558) or rabbit polyclonal antibodies (71-0700) in the H9c2 cells of controls with (lane 2) or without (lane 1) 20 μM EGCg pre-treatment for 30 min or 400 μM H2O2-induced oxidative stress for 30 min with (lane 4) or without (lane 3) 20 μM EGCg pre-treatment. Bottom: Quantitative analyses using β-actin as the loading control. In a and b, the values are the mean ± SEM (n = 6), with *, # indicating a significant difference compared to the untreated cells or the H2O2-treated cells, respectively.