Figure 5
Effects of H 2 O 2 and/or EGCg on the expression of Cav in H9c2 cells. (a) Agarose gels demonstrating the presence of mRNAs encoding different Cav isoforms (Cav-1, Cav-2, and Cav-3) (left), and a histogram showing the Cav isoform mRNA levels relative to those of GAPDH mRNA in cells exposed to 0.4 mM H2O2 with (EH) or without 20 μM EGCg pretreatment (H) (right). (b) Immunoblot analysis reporting the protein levels of Cav-1 and phosphorylated Cav-1in whole cell lysates of H9c2 cells cultured in the medium as indicated. GAPDH was used as the internal control for data analysis. In a and b, each value is the mean ± SEM (n = 6). *indicates significant difference compared to H9c2 cells in control condition (C), and # symbolizes a significant difference compared to cells treated with H2O2 (H).