Interaction of Plzf and Znf179 in yeast two-hybrid assay. ( A ) Schematic representation of Plzf clone that interacts with LexA-Znf179 (1–417) bait. N-terminal fragment of Znf179 was tagged at the N terminus with LexA. The Plzf fragment represents the clone isolated from the yeast two-hybrid screen of a mouse brain library linked to the Gal4 transactivation domain (Gal-AD). The first and last amino acids of the fragments are numbered with respect to their positions in full-length protein. ( B ) Yeast strain L40 was co-transformed with a bait (Znf179 N terminal fragment or the control protein lamin fused to the LexA protein) and a prey protein (Plzf fragment 180–673 fused to the Gal-AD or Gal-AD alone). Interaction was monitored by β-galactosidase activity assay using o-nitrophenylgalactoside or X-gal as substrates. Values represent the mean ± S.E.M. of at least three independent experiments. Statistical analysis was performed using one-way ANOVA with appropriate post hoc tests: ***P < 0.001.