Plzf interacts with Znf179 in vivo . ( A ) COS-1 cells were co-transfected with Flag-Plzf and EGFP-Znf179 vectors. Immunoprecipitation (IP) was performed with anti-Flag antibody or mouse IgG. The protein complex was then washed and analyzed by Western blotting as indicated. ( B ) P19 cells were transfected with Flag-Znf179 or vector only, then aggregation was induced in the presence of 1 μM all-trans-RA for two days. IP was performed with anti-Znf179 antibody. The protein complex was then washed and analyzed by Western blotting as indicated.