Effect of Znf179 on the transcriptional repression activity of Plzf. COS-1 cells were transfected with Gal4-Luc reporter construct (pFR-Luc) and Gal4-DBD-tagged Plzf or Znf179 together with Flag-Plzf, Flag-Znf179, or empty control vector. After 24 h transfection, the cells were collected and subjected to luciferase activity assay. The transfection efficiency was corrected by normalizing the data to the corresponding Renilla luciferase activity for each construct. Values of relative luciferase activity represent the mean ± S.E.M. of at least three independent experiments. Statistical analysis was performed using one-way ANOVA with appropriate post hoc tests: *P < 0.05 versus the control (empty vector) group. vector, empty control vector.