Effect of Znf179 on Plzf-mediated repression of Aurora kinase C promoter activity. SW480 cells were transfected with Aurora kinase C promoter construct and HA or HA-Plzf together with EGFP or EGFP-Znf179. After 24 h transfection, the cells were collected and subjected to luciferase activity assay. The transfection efficiency was corrected by normalizing the data to the corresponding Renilla luciferase activity for each construct. Values of relative luciferase activity represent the mean ± S.E.M. of at least three independent experiments. Statistical analysis was performed using Student’s t-test: *P < 0.05 versus the control (HA vector control) group.