Figure 4

Effects of dioscin on mineralization in MC3T3-E1 cells. Cells were treated with vehicle, dioscin (0.25 μg/ml, 0.5 μg/ml and 1.0 μg/ml) or lovastatin (0.04 μM) for 72 h. The medium was removed and cells were cultured with the medium supplemented with Vitamin C and β-glycerol phosphate disodium salt pentahydrate at final concentrations of 50 μg/ml and 10 mM at 37°C for 17 days. And the mineralized nodules were analyzed by von Kossa staining. (A) Control; (B) Dio 0.25; (C) Dio 0.5; (D) Dio 1.0; (E) Lov. **P < 0.01 vs Control.