Figure 4

The effect of HPB242 on downstream target proteins by the specificity protein 1 (Sp1). HN22 (A) and HSC4 (B) cells were incubated with HPB242 (0, 5, 10 and 20 μg/ml) for 48 h. The effect of HPB242 for 48 hours on p27, Cyclin D1, Mcl-1, and Survivin was determined by Western blots. Fifty micrograms of cellular extract per lane was separated on an SDS-PAGE gel as described in Methods. Equal loading of protein was confirmed by incubating the same membrane with anti-β-Actin antibody.