Figure 1
From: Structural and functional characterization of MERS coronavirus papain-like protease
Expression and purification of recombinant MERS-CoV PLpro. (A) Protein identification by SDS-PAGE. M: molecular marker. Lane 1–4: cytoplasmic fraction, flow-through, elution from the nickel affinity column and protein fraction from S-100 gel-filtration column. (B) and (C) Protein sequence identification by mass spectrometry. The PLpro was digested by trypsin and then analyzed by MALDI mass spectrometry. There are 15 matched peptides observed (B) and 60% sequence coverage are shown in bold red (C).