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Table 2 The kinetic parameters and DUB activity of MERS-CoV PLpro

From: Structural and functional characterization of MERS coronavirus papain-like protease

Proteins

Peptide cleavage

Deubiquitination

Km

k cat

kcat/Km

Activity (Intensity/s)b

(μM)a

(10-2 s-1)a

(10-3 s-1 μM-1)

MERS-CoV PLpro

    

Wild-type

19.2 ± 2.6

0.4 ± 0.02

0.2 ± 0.03

0.11 ± 0.02

L105W mutant

35.7 ± 3.8

16.5 ± 0.9

4.6 ± 0.6

0.11 ± 0.01

P162L mutant

30.8 ± 8.0

0.01 ± 0.001

0.003 ± 0.001

0.004 ± 0.001

SARS-CoV PLpro

25.2 ± 5.1c

11 ± 2c

4.4 ± 1.2c

0.12 ± 0.02

  1. aKinetic data of MERS-CoV PLpro and its mutants were fitted to the Michaelis-Menten equation (Eq. 1). The Rsqr were from 0.986 to 0.997, respectively. All the assays were repeated several times to ensure reproducibility.
  2. bFixed concentrations of Ub-AFC (0.5 μM) and PLpro (0.17 μM) were used.
  3. cThe values were from our previous studies [24].
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Journal of Biomedical Science

ISSN: 1423-0127