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Figure 4 | Journal of Biomedical Science

Figure 4

From: Sphingosine-1-phosphate promotes the differentiation of adipose-derived stem cells into endothelial nitric oxide synthase (eNOS) expressing endothelial-like cells

Figure 4

The effect of S1P on cell growth during differentiation of ASCs to the endothelial phenotype. Cell growth was evaluated by MTT assay (panel A), direct cell count (panel B) and EdU DNA-incorporation kit (panel C). Green staining indicates newly synthesized DNA with EdU incorporation and blue staining is DAPI staining to highlight nuclei. Four individual ASCs cell lines were used. Each cell line was subcultured into two identical sets, one for S1P treatment and one without S1P for control. Student t-test was performed to compare the difference between control and S1P treated cells. *indicated statistical significance with p < .05.

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