Effects of H
and EGCG on oxidative stress associated with cellular metabolism. (a) Measurements of intracellular ROS formation by DCF-DA in H9c2 cells (left). Cellular Ca2+ levels were measured using the Fura-2 fluorescence dye (right). (b) Representative result of Prdx4 protein levels in 2-DE gel and mRNA expression (c) Representative result of Eno1 protein levels in 2-DE gel and mRNA expression (d) Representative results of Aldh2, Oat and Sdha protein levels in 2-DE gel and mRNA expression. (e) ALDH activity was measured by spectrophotometer at 340 nm (left). Quantitative results showed protective effect of EGCG in H2O2-induced H9c2 cells (right). All values are the mean ± SEM, with *p < 0.05; **p < 0.01; ***p < 0.001.