Figure 5
Effects of H 2 O 2 and EGCG on PI3K/AKT/GSK3β signaling pathway. (a) Representative result of Inpp4b protein level in 2-DE gel and mRNA expression (b) Western blotting (right) and quantitative analysis (left) showing effects of EGCG on Inpp4b, p-AKT (S473), AKT, p-GSK-3β (S9), cyclin D1 in H2O2-induced H9c2 cells. (c) MTT assay showing the improvement of H2O2-suppressed cell viability by EGCG and/or GSK-3β inhibitor, SB216763 pre-treatment in H2O2-induced H9c2 cells. (d) Western blotting (right) and quantitative analysis (left) showing effects of EGCG and/or GSK-3β inhibition by GSK-3β inhibitor, SB216763, on the phosphorylation of Akt (S473), GSK-3β (S9) and cyclin D1 in H2O2-induced H9c2 cells. All values are the mean ± SEM, with *p < 0.05; **p < 0.01; ***p < 0.001.