Molecular identification for epigallocatechin-3-gallate-mediated antioxidant intervention on the H2O2-induced oxidative stress in H9c2 rat cardiomyoblasts

Table 2 List of identified differentially expressed proteins obtained with 2D-DIGE coupled with MALDI-TOF MS analysis

Spot no.	Swiss-Prot no.	Protein name	MW	pI	No. match peptides	Cov.(%)	Score	Function	H₂O₂/Ctrl	EGCG/H₂O₂
*Constitutively upregulated*
7	P04764	Alpha-enolase (Eno1)	47,440	6.16	18/48	43	153/51	Metabolism	+1.88	−1.77
184	Q68SB1	Double-stranded RNA-binding protein Staufen homolog 2 (Stau2)	62,870	9.54	14/61	23	59/51	RNA binding	+1.75	−2.46
239	Q9QWG5	Type II inositol 3,4-bisphosphate 4-phosphatase (Inpp4b)	106,205	5.86	12/44	14	57/51	Signal transduction	+2.24	−1.80
*Constitutively downregulated*
4	P11884	Aldehyde dehydrogenase, mitochondrial (Aldh2)	56,966	6.63	15/40	33	141/51	Metabolism	−1.99	+2.02
161	P61980	Heterogeneous nuclear ribonucleoprotein K (Hnrnpk)	51,230	5.39	10/39	28	76/51	RNA binding	−2.43	+2.01
221	P04182	Ornithine aminotransferase, mitochondrial (Oat)	48,701	6.53	13/38	30	123/51	Metabolism	−1.94	+1.87
259	Q9Z0V5	Peroxiredoxin-4 (Prdx4)	31,216	6.18	17/34	57	221/51	Antioxidation	−3.71	+2.89
261	Q920L2	Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (Sdha)	72,596	6.75	31/52	51	305/51	Electron transport	−3.21	+2.34

Average ratios of differential expression (p < 0.05) across H9c2 cells, H9c2 cells treated with and/or without 10 to 20 μM EGCG pre-treatment followed by 400 μM H₂O₂ exposure were calculated from triplicate gels.

ISSN: 1423-0127