Skip to main content

Table 2 List of identified differentially expressed proteins obtained with 2D-DIGE coupled with MALDI-TOF MS analysis

From: Molecular identification for epigallocatechin-3-gallate-mediated antioxidant intervention on the H2O2-induced oxidative stress in H9c2 rat cardiomyoblasts

Spot no. Swiss-Prot no. Protein name MW pI No. match peptides Cov.(%) Score Function H2O2/Ctrl EGCG/H2O2
  Constitutively upregulated         
7 P04764 Alpha-enolase (Eno1) 47,440 6.16 18/48 43 153/51 Metabolism +1.88 −1.77
184 Q68SB1 Double-stranded RNA-binding protein Staufen homolog 2 (Stau2) 62,870 9.54 14/61 23 59/51 RNA binding +1.75 −2.46
239 Q9QWG5 Type II inositol 3,4-bisphosphate 4-phosphatase (Inpp4b) 106,205 5.86 12/44 14 57/51 Signal transduction +2.24 −1.80
  Constitutively downregulated         
4 P11884 Aldehyde dehydrogenase, mitochondrial (Aldh2) 56,966 6.63 15/40 33 141/51 Metabolism −1.99 +2.02
161 P61980 Heterogeneous nuclear ribonucleoprotein K (Hnrnpk) 51,230 5.39 10/39 28 76/51 RNA binding −2.43 +2.01
221 P04182 Ornithine aminotransferase, mitochondrial (Oat) 48,701 6.53 13/38 30 123/51 Metabolism −1.94 +1.87
259 Q9Z0V5 Peroxiredoxin-4 (Prdx4) 31,216 6.18 17/34 57 221/51 Antioxidation −3.71 +2.89
261 Q920L2 Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (Sdha) 72,596 6.75 31/52 51 305/51 Electron transport −3.21 +2.34
  1. Average ratios of differential expression (p < 0.05) across H9c2 cells, H9c2 cells treated with and/or without 10 to 20 μM EGCG pre-treatment followed by 400 μM H2O2 exposure were calculated from triplicate gels.