Figure 5

Colocalization analysis of Cav-3 WT and Cav-3 V82I with the LB marker Nile red. Cav-3 transfected cells were labeled with Cav-3 antibodies followed by Alexa Fluor 488 conjugated secondary antibodies. LBs were then stained with Nile red (1:100 dilution in PBS from 1 mg/ml stock solution in acetone). BHK cells expressing Cav-3 V82I (bottom) showed a marked localization in LB structures (bottom) as compared to Cav-3 WT (top). Scale bar: 5 μm (2.5 μm in the inserts). Two independent transfected cultures were analyzed.