Table 2 Specific primers designed for the experiments
From: CITED2 Mutation and methylation in children with congenital heart disease
Species | Gene | Primer sequences | Amplified length |
|---|---|---|---|
Human | CITED2 | S1: Sense: 5'-AGGCTGTTAGTGGGATCTTGG -3' | 559 bp |
Anti: 5'-CATGTAGTGGTT- GTGGGGGTAG -3' | |||
S2: Sense: 5'-CCAGGTTTAACAACTCCCAGTTC-3' | |||
Anti: 5'-CCACAAGATTAAGCAGTTTGCC-3' | |||
BSP: Sense: 5'-GGTGGGGTAGATTTAGTTTGAGG-3' | 350 bp | ||
Anti: 5'-ACTTTAACCACAATTAATATAAACA TTTC -3' | |||
MSP: MF: 5’-CGCGTGGTGTTATACGGGACG -3’ | 199 bp | ||
MR: 5’-ACAAAACCTCCCTCCGAACT -3’ | |||
UF: 5’-TGTGTGGTGTTATATGGGATG-3’ | |||
UR: 5’-ACAAAACCTCCCTCCAAACT-3’ | |||
TFAP2c | Sense: 5'-AAATCCTTCTCCACCGCACAGACT-3' | 100 bp | |
Anti: 5'-TGATGCAGAACCAGTGAAGGCTCT-3' | |||
HIF-1α | Sense: 5'-CGTTCCTTCGATCAGTTGTC-3' | 143 bp | |
Anti: 5'-TCAGTGGTGGCAGTGGTAGT-3' | |||
β-actin | Sense: 5'-CATGGGTCAGAAGGATTCCTATGTG-3' | 116 bp | |
Anti: 5'-ATTTTCTCCATGTCGTCCCAGTTG-3' | |||
Rats | TFAP2c | Sense: 5'-GGATTTAACTGGCGACTAT-3' | 79 bp |
Anti: 5'-CCTCTTCATACTTGACATTATC-3' | |||
HIF-1α | Sense: 5'-CTGCCACCACTGATGAAT-3' | 128 bp | |
Anti: 5'-ACTGTATGCTGATGCCTTAG-3' | |||
β-actin | Sense: 5'- TATGGAATCCTGTGGCATC-3' | 87 bp | |
Anti: 5'-GTGTTGGCATAGAGGTCTT-3' |