Figure 2

Rechromatography of peak XI on CM Sephadex C-25 column. Peak XI (62 mg in 2 ml of 0.02 M phosphate buffer, pH7.0) rechromatographed on CM-Sephadex C-25 column (1.4×75 cm), pre-equilibrated with 0.02 M phosphate buffer, pH 7.0. Protein eluted in phosphate buffer of 0.02–0.15 M and pH 7.0–8.0. Fractions collected at 2.5 ml/5 min at 4°C, monitored at 280 nm. Peak III chosen for further purification.