Figure 5
PID15 induced apoptosis in isolated human peripheral lymphocytes. Lymphocytes (1×106 cells) ± PID-15 in 0.5 ml of HBSS, pH7.4, and reaction arrested in ice cooled bath. 2 μg of DNA from cells loaded on 0.8% agarose gel and electrophoresced. M. Hind III digest of λ-phage DNA molecular marker, 1.DNA isolated from untreated lymphocytes (1×106 cells). 2. DNA isolated from NV-PLA2 (1 μg protein) treated lymphocytes. 3. As in 2 + PID-15 (0.1 μmoles). 4. As in 2 + PID-15 (0.2 μmoles).