Figure 1
From: Antitumor effect of therapeutic HPV DNA vaccines with chitosan-based nanodelivery systems
Transfection analysis of the chitosan nanodelivery systems. A) CHO cells transfected by NCS – pEGFP; B) Western blot analysis of HPV E7 antigen expression by NCS-DNA E7. Cell extracts or supernatants were prepared as described in Methods; proteins were separated by SDS polyacrylamide gel electrophoresis, blotted on PVDF membranes, and incubated with the specific anti-HPV-16 E7 monoclonal antibody. The arrow indicates the E7 protein of approximately 11 KDa of molecule weight. Lane 1: Lysate from TC-1 cells, Lane 2: pcDNA3.1/E7 with Lipofectamine, lane 3: NCS-DNA E7.