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Table 2 Transcript and sequence of each primer used in real time RT-PCR and ChIP assays

From: NF-κB repressing factor downregulates basal expression and mycobacterium tuberculosis induced IP-10 and IL-8 synthesis via interference with NF-κB in monocytes

Transcript Sequence
For RT-PCR Primer
IP-10 F: 5′-AGTTAGCAAGGAAAGGTCT-3′
R: 5′-ACATTATAGTGCCAGGT-3′
IL-8 F: 5′-AGATCTGAAGTGTGATGACTCAGG-3′
R: 5′-GAAGCTTGTGTGCTCTGCTGTCTC-3′
NKRF F: 5′-AGAAAGATGGGTTGGACT-3′
R: 5′-CTGTGTGGCTCTCGGA-3′
GAPDH F: 5′-TTCCAGGAGCGAGATCCCT-3′
R: 5′-CACCCATGACGAACATGGG-3′
For ChIP assays Primer
NKRF promoter, F: 5′-AGGTTCAAGCAGTTTTCC-3′
R: 5′-CTGTAATCTCAGCACTTTGG-3′
IP-10 promoter F: 5′-AGGCTGGTCTCAAACT-3′
R: 5′-CCTCCCACATCCAATTACT-3′
IL-8 promoter F: 5′-GGGCCATCAGTTGCAAATC-3′
R: 5′-TTCCTTCCGGTGGTTTCTTC-3′
IP-10 3′-untranslated region (3′-UTR) F: 5′- TTGAGTTATAATTACTTAT-3′
R: 5′-TGAAAAGAAGGGTGAGAAGAG-3′
IL-8 3′-untranslated region (3′-UTR), F: 5′-AGGTTCAAGCAGTTTTCC-3′
R: 5′-CTGTAATCTCAGCACTTTGG-3′
  1. F = forward; R = reverse; IP-10 = Interferon gamma induced protein 10 kd; IL-8 = interleukin-8; NKRF = NF-κB repressing factor; ChIP = chromatin immunoprecipitation.