Table 2 Transcript and sequence of each primer used in real time RT-PCR and ChIP assays

For RT-PCR

Primer

IP-10

F: 5′-AGTTAGCAAGGAAAGGTCT-3′

R: 5′-ACATTATAGTGCCAGGT-3′

IL-8

F: 5′-AGATCTGAAGTGTGATGACTCAGG-3′

R: 5′-GAAGCTTGTGTGCTCTGCTGTCTC-3′

NKRF

F: 5′-AGAAAGATGGGTTGGACT-3′

R: 5′-CTGTGTGGCTCTCGGA-3′

GAPDH

F: 5′-TTCCAGGAGCGAGATCCCT-3′

R: 5′-CACCCATGACGAACATGGG-3′

For ChIP assays

Primer

NKRF promoter,

F: 5′-AGGTTCAAGCAGTTTTCC-3′

R: 5′-CTGTAATCTCAGCACTTTGG-3′

IP-10 promoter

F: 5′-AGGCTGGTCTCAAACT-3′

R: 5′-CCTCCCACATCCAATTACT-3′

IL-8 promoter

F: 5′-GGGCCATCAGTTGCAAATC-3′

R: 5′-TTCCTTCCGGTGGTTTCTTC-3′

IP-10 3′-untranslated region (3′-UTR)

F: 5′- TTGAGTTATAATTACTTAT-3′

R: 5′-TGAAAAGAAGGGTGAGAAGAG-3′

IL-8 3′-untranslated region (3′-UTR),

F: 5′-AGGTTCAAGCAGTTTTCC-3′

R: 5′-CTGTAATCTCAGCACTTTGG-3′