Figure 1
From: The difference in immune response and IL-12p35 methylation between newborns and adults
Interleukin-12 production in peripheral blood mononuclear cells from adults and neonates. Peripheral blood mononuclear cells from adults (n = 8) or neonates (n = 10) were cultured in a 48-well plate (2 × 106 cells/ml) in the presence of 100 ng/ml of lipopolysaccharide. After 24 hours of stimulation, IL-12 production was evaluated in the culture supernatants using enzyme-linked immunosorbent assay. The results, obtained by subtracting the control values from the stimulated values, are expressed in pg/ml.