Figure 2

Impaired IL-12p35 mRNA production in lipopolysaccharide (LPS)-stimulated neonatal peripheral blood mononuclear cells (PBMCs). (a) IL-12p35 mRNA levels were determined by real-time PCR. The PBMCs were incubated with medium alone or stimulated with LPS (1 ng/ml) for 3 hours and 6 hours. IL-12p35 mRNA levels were normalized using endogenous control GAPDH and compared with the non-stimulated group. (b) The PBMCs were incubated with medium alone or stimulated with LPS (1 ng/ml) for the indicated period of time. IL-12p40 mRNA levels were assessed as described in (a). The results are representative of at least 3 independent experiments performed with different donors.