Autophagic flux was induced in EV71-infected SK-N-SH cells. SK-N-SH cells were infected with EV71 for various times at MOI of 10. Cells infected with EV71 were treated with or without ammonium chloride NH4Cl (2.5 mM) for 1 hr, and the expression levels of EV71 VP1 and LC3-II were evaluated by Western blotting using specific antibodies. β-actin was used as the internal control. The numbers under each band represent the intensity of each band measured by densitometric analysis using VisionWorksTM LS image acquisition and analysis software (UVP, Upland, CA, USA). For the comparison of LC3-II levels, we set the intensity of Mock at each time point as 1 (normalized with the intensity of β-actin). For the comparison of VP1 levels, we set the intensity of EV71 infection without NH4Cl as 1 at each time point (normalized with the intensity of β-actin).