Figure 2

Autophagic flux was induced in EV71-infected SK-N-SH cells. SK-N-SH cells were infected with EV71 for various times at MOI of 10. Cells infected with EV71 were treated with or without ammonium chloride NH₄Cl (2.5 mM) for 1 hr, and the expression levels of EV71 VP1 and LC3-II were evaluated by Western blotting using specific antibodies. β-actin was used as the internal control. The numbers under each band represent the intensity of each band measured by densitometric analysis using VisionWorks^TM LS image acquisition and analysis software (UVP, Upland, CA, USA). For the comparison of LC3-II levels, we set the intensity of Mock at each time point as 1 (normalized with the intensity of β-actin). For the comparison of VP1 levels, we set the intensity of EV71 infection without NH4Cl as 1 at each time point (normalized with the intensity of β-actin).