miRNA
|
miRNA expression1
|
Target transcript
|
Protein expression2
|
---|
(Mean ± SD)
|
(Relative to normal)
|
---|
let-7d-5p
|
2.29 ± 1.01
|
IGF1R
|
0.33 ± 0.23** (α)
|
| | |
0.53 ± 0.37** (β)
|
let-7d-3p
|
−2.78 ± 0.79**
|
KRAS
|
3.57 ± 1.99**
|
- The colon cancer cells lines used were HT-29, SK-CO-1 and WiDr. 1Real-time PCR was used to determine miRNA and target mRNA levels; the log2 (fold change) values are shown. 2Western blotting was used to determine protein levels and the values were expressed relative to those of normal tissues. In both real-time PCR and western analysis, data were derived from three independent experiments. **p < 0.01.