MicroRNA-5p and -3p co-expression and cross-targeting in colon cancer cells

Table 5 Expression levels of let-7d-5p and -3p and associated putative target transcripts and proteins in colon cancer cell lines

miRNA	miRNA expression¹	Target transcript	Protein expression²
miRNA	(Mean ± SD)	Target transcript	(Relative to normal)
let-7d-5p	2.29 ± 1.01	IGF1R	0.33 ± 0.23** (α)
			0.53 ± 0.37** (β)
let-7d-3p	−2.78 ± 0.79**	KRAS	3.57 ± 1.99**

The colon cancer cells lines used were HT-29, SK-CO-1 and WiDr. ¹Real-time PCR was used to determine miRNA and target mRNA levels; the log₂ (fold change) values are shown. ²Western blotting was used to determine protein levels and the values were expressed relative to those of normal tissues. In both real-time PCR and western analysis, data were derived from three independent experiments. **p < 0.01.

ISSN: 1423-0127