Figure 2

Effects of miR-466 and miR-4305 on Prox1 expression. (A) Reduction in Prox1 mRNA levels by miR-466. HDLEC were transfected with the miR-181a, miR-466, miR-4305 mimics, or the scrambled control. The cells were then cultured for 48 h and harvested for use in real-time qRT-PCR analyses (n = 3). Error bars indicate SDs. †P < 0.01. (B) Effects of miR-466 and miR-4305 on the expression of Prox1 protein. HDLEC were transfected with miR-181a, miR-466, miR-4305 mimics, or the scrambled control. The cells were cultured for 48 h and then harvested for analysis. The band densities obtained using an anti-Prox1 antibody were divided by those obtained using an anti–beta-actin antibody to normalize protein loading. Western blotting was conducted using three sets of independently transfected HDLEC. Bands were quantified using Fujifilm Multi Gauge software (version 3.0). Means were calculated using data from all three independent experiments and are expressed as ratios to the value obtained from the scrambled control-transfected HDLEC. Error bars indicate SDs. *P < 0.05. †P < 0.01.