Figure 1

Representative 2-D gel map of the hepatic proteins in apoE-KO mice. Mice were fed a chow diet (control) ad libitum and animals in the experimental groups were fed with chow diet plus 200 mg/kg of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), arachidonic acid (AA), or linoleic acid (LA) in 1.1% ethanol/PBS every day by gavage. The proteins extracted from the liver were separated initially using a pH 5–8 IPG strip as the first dimension and then on a 12.5% SDS-polyacrylamide gel as the second dimension. The gels were stained with Coomassie blue G-250 and molecular weight standards are shown on the left. The differentially modulated protein spots are numbered and their identities are reported in Table 2.