Differential regulation of protein expression in response to polyunsaturated fatty acids in the liver of apoE-knockout mice and in HepG2 cells

Huang, Chun-Ying; Chen, Wei-Ming; Tsay, Yeou-Guang; Hsieh, Shu-Chen; Lin, Yun; Lee, Wen-Jane; Sheu, Wayne Huey-Herng; Chiang, An-Na

doi:10.1186/s12929-015-0118-2

Journal of Biomedical Science

Table 2 Summary of the regulated proteins present in the liver of apoE KO mice treated with different PUFAs

From: Differential regulation of protein expression in response to polyunsaturated fatty acids in the liver of apoE-knockout mice and in HepG2 cells

Spot no. ^(a)	Protein ^(b)	Ratio PUFA/control ^(c)	Accession number ^(b)
	Metabolism
D13	Fumarylacetoacetase	3.3	gi\|544273
D14	Fructose-1,6-bisphosphatase 1	2.9	gi\|14547989
D17	Malate dehydrogenase	2.6	gi\|126889
D20	Oxysterols receptor LXR alpha	2.3	gi\|12644486
D25	Aldehyde dehydrogenase	2.1	gi\|1352250
D26	Isocitrate dehydrogenase	2.1	gi\|6647554
D31	SREBP-1	0.5	gi\|7144550
D32	Pyruvate carboxylase	0.5	gi\|6679237
E8	ATP synthase beta chain	3.1	gi\|20455479
E9	Malate dehydrogenase	2.8	gi\|126889
E10	Isocitrate dehydrogenase	2.6	gi\|6647554
E22	Alpha enolase	2.0	gi\|13637776
E23	Fructose-1,6-bisphosphatase 1	2.0	gi\|14547989
E24	Pyruvate carboxylase	0.5	gi\|6679237
E25	SREBP-1	0.5	gi\|7144550
A10	Pyruvate kinase isozyme M2	2.6	gi\|2506796
A12	Malate dehydrogenase	2.2	gi\|126889
A13	Glycerol-3-phosphate dehydrogenase	2.1	gi\|121557
A14	Ketohexokinase	2.1	gi\|6016435
L3	Succinyl-CoA synthetase	4.8	gi\|52788305
L9	Fructose-1,6-bisphosphatase 1	3.4	gi\|14547989
L10	Fumarylacetoacetase	3.2	gi\|544273
L14	Phosphoglucomutase	2.8	gi\|21362784
L27	Vitamin D-binding protein precursor	2.0	gi\|46397761
	Redox stress/Inflammation
D1	Annexin A2	8.2	gi\|13435564
D6	Interleukin 1 receptor accessory	4.2	gi\|19882203
D9	Peroxiredoxin 6	3.7	gi\|6671549
D10	GlutathioneS-transferase, pi1	3.5	gi\|10092608
D15	MAP kinase kinase 3	2.9	gi\|1771303
D21	A-kinase anchor protein	2.3	gi\|2852699
D22	Glutathione S-transferase Mu 5	2.3	gi\|1346207
D23	Electron transfer flavoprotein alpha-subunit	2.2	gi\|21759113
D24	DC-SIGN related protein 1	2.2	gi\|46395849
D30	Selenium bind protein 2	0.5	gi\|9507079
E1	Selenium bind protein 2	6.9	gi\|9507079
E6	Peroxiredoxin 6	3.6	gi\|3219774
E7	G-protein coupled receptor	3.1	gi\|460318
E12	Interleukin 6 receptor, alpha	2.5	gi\|7110655
E13	Glutathione S-transferase theta 2	2.4	gi\|81916034
E16	Glucocorticoid-attenuated response gene 49 protein	2.2	gi\|6831574
E18	Peroxiredoxin 4	2.1	gi\|3024715
A5	Superoxide dismutase 1, soluble	4.7	gi\|45597447
A11	Cyclophilin A	2.3	gi\|118105
A20	Selenium bind protein 2	0.5	gi\|9507079
L4	Glutathione peroxidase 1	4.7	gi\|84871986
L6	Interferon regulatory factor	3.6	gi\|972949
L7	Adrenoleukodystrophy protein	3.6	gi\|6651050
L8	Glutathione S-transferase Mu 1	3.4	gi\|121716
L15	Glutathione transferase omega-1	2.7	gi\|6016174
L19	Heat shock protein 60	2.4	gi\|51702252
L20	Mitogen-activated protein kinase 10	2.3	gi\|2499604
L23	Farnesoid X-activated receptor	2.2	gi\|21263825
L25	Peroxiredoxin 4	2.1	gi\|3024715
	Enzyme
D2	Glycine N-methyltransferase	7.1	gi\|6754026
D5	DEAD-box RNA helicase	4.3	gi\|6014946
D7	Cyp2c70 protein	3.8	gi\|19387996
D8	3’-phosphoadenosine-5’-phosphosulfate synthase 1	3.7	gi\|6754982
D16	Isopentenyl-diphosphate delta-isomerase 1	2.7	gi\|13878548
D19	Indolethylamine N-methyltransferase	2.4	gi\|731019
D27	Homogentisate 1,2-oxygenase	2.1	gi\|7387755
D28	Thiopurine S-methyltransferase	2.0	gi\|6094505
E2	Dehydrodolichyl diphosphate synthase	5.9	gi\|46395956
E20	Thiopurine S-methyltransferase	2.1	gi\|6094505
A17	Ubiquitin carboxyl-terminal hydrolase L1	0.4	gi\|20178168
A19	Hypoxanthine guanine phosphoribosyl transferase 1	0.5	gi\|13435621
L2	Amine N-sulfotransferase	5.1	gi\|81870419
L11	3-hydroxyanthranilate 3,4-dioxygenase	2.9	gi\|3929397
L12	Caramoyl-phosphate synthase I	2.8	gi\|117492
L17	Era-like-GTPase	2.5	gi\|19852066
L18	Betaine-homocysteine S-methyltransferase	2.5	gi\|5915784
L26	Thiopurine S-methyltransferase	2.0	gi\|6094505
	Miscellaneous
D3	Hypothetical protein LOC74919	6.8	gi\|33563309
D4	Chaperonin groEL precursor	5.4	gi\|72957
D11	EF hand domain	3.4	gi\|13386360
D12	Growth-arrest-specific protein 2	3.4	gi\|120945
D18	Glial fibrillary acidic protein	2.6	gi\|417050
D29	Protein disulfide-isomerase A6 precursor	0.4	gi\|2501206
E3	RB-associated-KRAB repressor	5.1	gi\|29789126
E4	Hypothetical protein DKFZp547P193.1	4.3	gi\|11346331
E5	Myc-binding protein-associated protein	3.7	gi\|24762234
E11	SEC63	2.6	gi\|31981948
E14	START domain	2.4	gi\|9910482
E15	Growth-arrest-specific protein 2	2.2	gi\|120945
E17	Alpha-soluble NSF attachment	2.2	gi\|17380315
E19	Beta-actin	2.1	gi\|46397316
E21	Selenium bind protein 1	2.1	gi\|22164798
A1	Procollagen, type XXIII, alpha 1	9.8	gi\|23510253
A2	Advillin	8.3	gi\|6857753
A3	Putative TDPOZ1 protein	7.7	gi\|38077962
A4	Serum albumin precursor	7.2	gi\|5915682
A6	Hypothetical protein LOC225913	4.5	gi\|21703976
A7	Growth-arrest-specific protein 2	3.4	gi\|120945
A8	Beta-actin	3.1	gi\|46397316
A9	START domain	2.8	gi\|9910482
A15	Chimerin 1 isoform 2	0.4	gi\|13386436
A16	Rab GDP dissociation inhibitor beta	0.4	gi\|1707891
A18	Rho GDP dissociation inhibitor 1	0.4	gi\|21759130
L1	Upp2 protein	5.3	gi\|20071298
L5	Eukaryotic translation initiation factor 3 subunit 2	3.9	gi\|20138778
L13	Growth-arrest-specific protein 2	2.8	gi\|120945
L16	Regucalcin	2.6	gi\|2498920
L21	Caspase-6 precursor	2.3	gi\|2493529
L22	Guanosine diphosphate dissociation inhibitor 2	2.2	gi\|13638229
L24	Transducin alpha-1 chain	2.1	gi\|121033
L28	Apolipoprotein A-IV precursor	2.0	gi\|1703331

(a) The spot numbers correspond to the same protein signal that was detected in the Figure 1.
(b) The protein names and accession numbers were taken from the NCBI database.
(c) Ratio of the protein intensity in each PUFA-treated group to the corresponding spot in the control group represents average values of triplicate samples.

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ISSN: 1423-0127

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