Figure 1From: Shikonin selectively induces apoptosis in human prostate cancer cells through the endoplasmic reticulum stress and mitochondrial apoptotic pathway Shikonin treatment selectively induces apoptosis in prostate cancer cells. (A) Shikonin treatment inhibits prostate cancer cell viability without affecting normal prostate epithelial cells. DU-145, PC-3 and PrEC cells were treated with various doses (0.5, 1, 2.5, 5 and 10 μM) of Shikonin for 24 h and CCK-8 assay was used for the measurement of cell viability. (B) Shikonin treatment induces DNA fragmentation in prostate cancer cells. Cells were treated with various doses (0.5,1,2.5, 5 and 10 μM) of Shikonin for 24 h and TUNEL assay was done using flow cytometry and the % TUNEL positive cells were measured. (C) Shikonin treatment induces PARP activation. Cells were treated with 2.5 μM of Shikonin for 24 h and the cell lysates were analyzed by western blotting for the detection of cleaved PARP, a marker for PARP activation. (D) Shikonin treatment induces caspase-3 activation. Cells were treated with various doses of Shikonin (0.5, 1, 2.5, 5 and 10 μM) for 48 h and measurement of caspase-3 activity was done using the CASPASE-3 Colorimetric Activity Assay kit. Data is expressed in means ± SEM and represents the results of three independent experiments (p < 0.05).Back to article page