Figure 4

Direct cell conversion by Oct4. a) Immunofluorescence of Oct4 at 24 h after infection. b) Representative picture of the appearance of the mutated colonies at day 7–10 after Oct4 infection. c) Small cells that rapidly expanded from the emerging colonies. d) Representative image of flow cytometry of a six-cell-mutated clone at day 14, showing the emergence of CD34+ and CD45+ cells in Oct4 transduced cells as compared to the six-cell-mutated control.