Subcellular distribution of MSP58. A. Immunocytochemical staining of HeLa cells using a MSP58 polyclonal antibody. MSP58 was visualized using a secondary antibody conjugated to FITC (green), and nuclei were counterstained with DAPI (blue). Nucleoli are visible as dark regions in the nuclei (arrowhead). Note the presence of several microspherules in the nucleoli. B. Immunohistochemical staining of MSP58 protein expression in a tissue of human oral cavity squamous cell carcinoma (OCSCC). Images were analyzed using an Olympus BX51 microscope with 200× magnification. Arrowheads indicate nucleoli.