Fig. 8

The icv-MPP⁺-injection-induced activation of radial glial-like stem cells and new-borne neurons in subgranular zone were attenuated by icv-LSA-injection. Two μl vehicle, MPP⁺ (12 μg) alone or MPP⁺ (12 μg) combined with LSA (5 μg) were injected into lateral ventricle of ICR mice. The mice were sacrificed 5 days later. Brain of the mice was subjected to cryosection and immunostained by anti-GFAP antibody (green) and anti-doublecortine antibody (DCX, red). Upper panel is the representative fluorescence images. Lower panel is the quantification of GFAP-labeled radial glial-like cells and DCX-positive cells . Results are means ± S.D. from three independent experiments. Significant differences between the mice treated with vehicle and MPP⁺ were indicated by ***, P < 0.001. Significant differences between the mice treated with MPP⁺ alone and MPP⁺ combined with LSA were indicated by ###, P < 0.001