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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: In vivo imaging of endogenous enzyme activities using luminescent 1,2-dioxetane compounds

Fig. 6

CIEEL energy transfer imaging of elevated alkaline phosphatase activity during tissue inflammation. a Splenocytes were harvested and cultured in the presence of LPS, a potent stimulant for inflammatory responses. The cells were then stained with DiR and subjected to CIEEL imaging with Galacton-plus. Untreated cells were used as negative control. The cells were scanned from 520–800 nm to establish their luminescence emission profiles. b CIEEL imaging of endogenous phosphatase activity in the peritoneal cavity of normal mice. Animals received i.p. injection of CSPD in the presence or absence of DiR, and were then subject to sequential luminescence acquisition from 520 to 800 nm. c NCr nude mice were i.p. treated with LPS to induce inflammatory responses in the peritoneal cavity. CIEEL imaging was performed 24 h later. Untreated control mice were used as imaging control. d Mice were pretreated with dexamethasone, a potent anti-inflammatory drug, and then challenged by i.p. LPS injection. CSPD-DiR CIEEL imaging was performed 24 h after LPS challenge. Error bar = SEM

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