Fig. 5

Relative signal pathways were determined by Western blot. There were not significant changes in the expressions of Akt, mTOR, MER1/2 and Erk1/2 in P-S (A549 and 95D) comparing with their control counterparts respectively. SLC34A2-tranfected A549 cells exhibited reduced expression levels of PI3K, p-Akt, p-mTOR, p-MEK1/2, p-Erk1/2 and CyclinD3. No significant changes in expression levels of PI3K, p-Akt, p-mTOR and CyclinD3 were found between SLC34A2- and vector- transfected 95D and 95D cells, whereas expressions of p-MEK1/2 and p-Erk1/2 in SLC34A2-transfected 95D cells were reduced (Cells transfected with pcDNA3.1-SLC34A2 or pcDNA3.1 were named as P-S or P respectively. Untransfected cells were named as Control.)