Table 1 Primers used in qRT-PCR
From: Investigating the potential of Shikonin as a novel hypertrophic scar treatment
Protein name | Corresponding gene name | Primers: Forward (F) & Reverse (R) |
|---|---|---|
Caspase 3 | CASP3 | F: 5’-CGGAAGCAGTGCAGACGCGG-3’ |
R: 5’-GCTGCGAGCACTCACGAAACTCTTC-3’ | ||
Bcl-2 | BCL2 | F: 5’-TCAACCGGGAGATGTCGCCCC-3’ |
R: 5’-ACAAAGGCATCCCAGCCTCCGTT-3’ | ||
Bax | BAX | F: 5’-AAACTGGTGCTCAAGGCCC-3’ |
R: 5’-TGAGGAGTCTCACCCAACCA-3’ | ||
Cytochrome c | CYCS | F: 5’-ATGGTCTCTTTGGGCGGAAG-3’ |
R: 5’-CTCCCCAGATGATGCCTTTGT-3’ | ||
Collagen I | COL1A1 | F: 5’-ACGAAGACATCCCACCAATC-3’ |
R: 5’-AGATCACGTCATCGCACAAC-3’ | ||
Collagen III | COL3A1 | F: 5’-GCCTCCCGGAAGTCAAGGAGAAAG-3’ |
R: 5’-CTTTAGGACCGGGGAAGCCCATG-3’ | ||
αSMA | αSMA | F: 5’-CTGCTGAGCGTGAGATTGTC-3’ |
R: 5’-CTCAAGGGAGGATGAGGATG-3’ | ||
GAPDH | GAPDH | F:5’-TCTTTTGCGTCGCCAGCCGAG-3’ |
R:5’-TGACCAGGCGCCCAATACGAC-3’ |