Fig. 3

Expression levels of markers of DNA damage and apoptosis. TW1 and TW4 cells were treated with 60 and 80 μM NEO212, and cellular lysates were harvested 1, 2 or 3 days later. In parallel, untreated cells were harvested after 1 day, and vehicle-treated cells (vh.) were collected after 3 days. Western blot analysis was performed with antibodies specific for full-length (f.l.) and cleaved (cl.) PARP, cleaved caspase 7, and p-H2AX, with actin as the loading control