Table 1 Characteristics of the primers used in the Semiquantitative RT-PCR of connexins
From: Analysis of connexin expression during seizures induced by 4-aminopyridine in the rat hippocampus
Gene | Primer sequence forward and reverse | Nucleotide position | NCBI data base ref | Expect product (bp) | Hybridization temperature (°C) |
|---|---|---|---|---|---|
C × 32 | 5′-CGGCATCTGCATTATCCTCAAC-3′ | 643-664 | BC078868.1 | 163 | 57 |
5′-CAGCAGCTTGTTGATCTCATTCTG-3′ | 782-805 | ||||
C × 36 | 5′-GCAGAGAGAACGCCGGTACT-3′ | 424-443 | Y16898 | 270 | 57 |
5′-CTTGGACCTTGCTGCTGTGC-3′ | 660-679 | ||||
C × 43 | 5′-GATGAGGAAGGAAGAGAAG-3′ | 493-511 | AY324140 | 620 | 57 |
5′-CGCTAGCTTGCTTGTTGTAA-3′ | 1096-1105 | ||||
β-actin | 5′-GCAAGAGAGGTATCCTGACC-3′ | 260-279 | BC063166.1 | 318 | 57 |
5′-CCCTCGTAGATGGGCACAGT-3′ | 565-584 |