Fig. 1

5’-AMP induced whole body cooling increases ΔF508-CFTR expression levels in CF mouse intestine. Representative cross-sections of mouse duodenal crypts from WT, untreated CF and treated CF mice were immunostained with anti-CFTR rabbit polyclonal antibody (red) and counter stained with DAPI (blue): a Treated CF mice that have undergone one treatment of 5’-AMP whole body cooling. b Treated CF mice that have undergone two treatment cycles. CF mice were sacrificed post-treatment at 24 h, 48 h and six days for tissue analysis. Quantification of ΔF508-CFTR expression was based on either positive or negative signals in intestinal goblet cells in equivalent visual fields in the respective genotype and treatment group