Fig. 4
From: FcγRIIB mediates antigen-independent inhibition on human B lymphocytes through Btk and p38 MAPK
FcγRIIB crosslinking reduces the number of antibody-secreting PCs resulting from BCR-independent activation of B cells. Purified B cells were treated with CpG (5 μg/ml) without additional antibody or in the presence of an isotype matched control mAb or AT10 mAb in graded concentrations (0, 1, 2.5, 5 or 10 μg/ml) for five days. ELISPOT analyses were performed to measure total IgM- and IgG-antibody-secreting PCs. Data are shown as an average of all individuals analyzed (top) or for individual donors (bottom). The asterisks indicate that the differences between the groups compared are statistically significant (P < 0.05)