Fig. 1

ROS generation and oxidative stress pathways are involved in aging. A- ROS production was assessed by DHE, DCF, DAF and HPF staining. Top panel shows representative images of HSCs; the aged group presented change in the number of cells that stained ROS-positive compared with young animals. Bar graph shows the difference on MFI of the ROS measured by flow cytometry among the groups. Values are means ± SEM, *p < 0.05 vs. young. ^# p < 0.05 vs. middle