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Table 1 PCR primers used to amplify wild-type and deletion mutants of UL76. The size of products were list on the right. Restriction endonuclease sites are underlined in the primer sequences

From: Unconserved C terminal of human cytomegalovirus tegument protein pUL76 elicits nuclear aggresome formation and induces DNA damage in transfected cells

  Primers Product size/bp
UL76(1-325aa) F: 5′-AAGCTTATGCCGTCCGGGCGT-3′ 975
R:5′-GGATCCGCTAAAGACCGTGTGGGACGGCG-3′
UL76N(1-190aa) F:5′- AAGCTTATGCCGTCCGGGCGT-3′ 570
R:5′- GGATCCGCCCGTCCCAGATAGTC-3′
UL76C(187-325aa) F:5′-AAGCTTATGGACTATCTGGGACGGCG-3′ 420
R:5′- GGATCCGCTAAAGACCGTGTGGGACGGCG-3′