Table 1 PCR primers used to amplify wild-type and deletion mutants of UL76. The size of products were list on the right. Restriction endonuclease sites are underlined in the primer sequences
Primers | Product size/bp | |
|---|---|---|
UL76(1-325aa) | F: 5′-AAGCTTATGCCGTCCGGGCGT-3′ | 975 |
R:5′-GGATCCGCTAAAGACCGTGTGGGACGGCG-3′ | ||
UL76N(1-190aa) | F:5′- AAGCTTATGCCGTCCGGGCGT-3′ | 570 |
R:5′- GGATCCGCCCGTCCCAGATAGTC-3′ | ||
UL76C(187-325aa) | F:5′-AAGCTTATGGACTATCTGGGACGGCG-3′ | 420 |
R:5′- GGATCCGCTAAAGACCGTGTGGGACGGCG-3′ |