Fig. 4
From: Activation of MEK2 is sufficient to induce skin papilloma formation in transgenic zebrafish
Skin tumor formation in Tg(krt14: MEK2S219D-GFP) zebrafish. Skin cells proliferated and formed papilla from 3dfp (b, B’, white arrow) on the yolk skin in Tg(krt14: MEK2S219D-GFP) embryos to form a skin tumor at 6 dpf (c, and C’; lateral view, d and D’, white-dots area; ventral view, e and E’, white-dots area). Tg(krt14:GFP) embryos had developed normal skin cells at 6 dpf (a, A’). An immunohistochemical experiment was used to detect ERK phosphorylation in skin tumors. p-ERK was detected by a p-ERK monoclonal antibody and visualized by DAB (brown). Nuclei were counterstained with hematoxylin (blue) (f, F’)