High fat diet-induced inflammation and oxidative stress are attenuated by N-acetylneuraminic acid in rats

Table 2 Names, accession numbers and primer sequences used in the study

Name	Left sequence	Right sequence
nfkb1	AGGTGACACTATAGAATACACTCCATATTTAATGCAGA	GTACGACTCACTATAGGGAGAAATCCTCTCTGTTTCG
B2m^a	AGGTGACACTATAGAATAATGCTTGCAGAGTTAAACA	GTACGACTCACTATAGGGATGCATAAAATATTTAAGGTAAGA
Hprt1^{a, b}	AGGTGACACTATAGAATATCCTCATGGACTGATTATG	GTACGACTCACTATAGGGACTGGTCATTACAGTAGCTCTT
Rpl13a^a	AGGTGACACTATAGAATAATGGGATCCCTCCAC	GTACGACTCACTATAGGGAATTTTCTTCTCCACATTCTT
Kan(r)^c
Sod1	AGGTGACACTATAGAATAATATGGGGACAATACACAA	GTACGACTCACTATAGGGATCCAACATGCCTCTCT
Sod2	AGGTGACACTATAGAATACAGGTTGCTCTTCAGC	GTACGACTCACTATAGGGAAACTCTCCTTTGGGTTCT
Gpx1	AGGTGACACTATAGAATATTGAGAAGTTCCTGGTAGGT	GTACGACTCACTATAGGGATTTTCTGGAAATCAGGTGT
Gsr	AGGTGACACTATAGAATAAATAAACTGGGGATTCAGAC	GTACGACTCACTATAGGGAAGTAGATTTTCACATTGTCTTTG
CRP	AGGTGACACTATAGAATACTAAACAGGCCTTCGTATT	GTACGACTCACTATAGGGACAAGCCAAAGCTCTACAAT

^aHousekeeping genes. ^bNormalization gene. Underlined sequences are left and right universal sequences (tags). ^cinternal control supplied by Beckman Coulter Inc (Miami, FL, USA) as part of the GeXP kit. RT conditions were: 48 °C for 1 min; 37 °C for 5 min; 42 °C for 60 min; 95 °C for 5 min, then hold at 4 °C. PCR conditions were initial denaturation at 95 °C for 10 min, followed by two-step cycles of 94 °C for 30 s and 55 °C for 30 s, ending in a single extension cycle of 68 °C for 1 min

ISSN: 1423-0127