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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: SB203580 increases G-CSF production via a stem-loop destabilizing element in the 3’ untranslated region in macrophages independently of its effect on p38 MAPK activity

Fig. 2

Effects of various p38 MAPK inhibitors on LPS-induced G-CSF mRNA in RAW264.7 and THP-1 macrophages. RAW264.7 cells (a) and THP-1 (b) were pretreated for 30 min with DMSO or 10 μM SB203580, SB202190, PD169316, SB239063, or SKF86002, then 100 ng/ml of LPS was added and incubation continued for 6 h, then the G-CSF mRNA levels were determined by RT-qPCR, normalized to GAPDH mRNA levels, and expressed relative to the value in the DMSO control, set as 1. The values are the mean ± SD for three separate experiments. *p < 0.05, compared to the DMSO-treated cells. (c) THP-1 macrophages were left untreated (left lane) or were pretreated for 30 min with DMSO or 10 μM SB203580, SB202190, PD169316, SB239063, or SKF86002, then 100 ng/ml of LPS was added and incubation continued for 30 min, then levels of phosphorylated HSP27, phosphorylated p38 MAPK, and total p38 MAPK were determined by Western blotting

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Journal of Biomedical Science

ISSN: 1423-0127