Fig. 3

Ethanol does not induce apoptosis or autophagy in basal progenitors. a Cells were exposed with 4 mg/ml ETOH for 8, 12 and 24 h and subsequently percentage viability was determined using Vi-Cell analyzer. b Percentage viability for chronic intermittent ethanol exposure (CIE) model. Data were analyzed by one-way analysis of variance (ANOVA) and Newman-Keul’s posthoc test and no significant difference was noted among the groups. c Cytotoxic effect of ethanol was assessed using ApoTox-Glo Triplex assay kit and the graphs represent relative fluorescence unit from dead cells. d Equal amount of protein lysates from either control or 12 and 24 h ETOH treated samples were subjected to immunoblot analysis for detection of activation of caspase-3 (cleaved caspase). Tubulin was used as a loading control. e Western blot analysis was performed on cell lysates treated with or without ETOH for 12 and 24 h to determine the protein levels of autophagosome initiation marker, beclin 1. Bottom panel demonstrates relative intensity of beclin 1 over loading control tubulin. A representative immunoblot image is provided for panels d and e. For panel c and e, Student’s t-test was performed and ns indicate not significant when compared with control