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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Therapeutic evaluation of HIV transduction basic domain-conjugated superoxide dismutase solution on suppressive effects of the formation of peroxynitrite and expression of COX-2 in murine skin

Fig. 1

Measurement of heavy metals in Tat-SOD using an ICP-AES and circular dichroism analysis of Tat-SOD. a Overexpression of the antioxidant Cu,Zn-SOD gene in an E. coli expression system. Tat-SOD was expressed in a metal-dependent manner. b Identification the contents of copper, zinc, and arsenate in E. coli expressed Tat-SOD by incubating with 0, 20, 40, 60 or 80 μM of arsenic ions as indicated were analyzed by ICP-AES. c Identification the contents of copper, zinc, and mercury in E. coli expressed Tat-SOD by incubating with 0, 4, 8, 12, 16 or 20 μM of mercury ions as indicated were analyzed by ICP-AES. d Effects of arsenate and mercury on the secondary conformation of the SOD protein using a circular dichroism analysis (with a Jasco J-715 spectrometer)

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Journal of Biomedical Science

ISSN: 1423-0127