Fig. 5

CNGRC-yCD/5-FC combination treatment significantly reduces cell viability of high-APN-expressing cells. a-b. HT-1080 a. and HT-29 cells b. were treated with 2.0 μM of proteins and with an increasing dose of 5-FC (0.1, 1.0, 10, 100 and 1000 μM). Then, the cell viability was determined by MTT assay. c-d. High-c and low-d APN-expressing cells were treated with 2.0 μM of protein and 60 μM of 5-FC and then the cell viability was determined by MTT assay. Treatments with 5-FC and 5-FU alone were used as negative and positive controls, respectively. At least three repeats were performed with similar results (*, t test, P < 0.05 relative to the 5-FC treatment group; #, t test, P < 0.05 relative to the yCD/5-FC treatment group)