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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Betulinic acid enhances TGF-β signaling by altering TGF-β receptors partitioning between lipid-raft/caveolae and non-caveolae membrane microdomains in mink lung epithelial cells

Fig. 4

BetA enhances TGF-β-induced Smad2 phosphorylation and nuclear translocation in Mv1Lu cells. Cells were pretreated with BetA, for 0, 0.5, 1, 2, 4, and 6 h, and then further incubated with 100 pM TGF-β for 30 min. The P-Smad2 and total Smad2 levels in the cell lysates were analyzed by immunoblotting. The relative level of P-Smad2 (P-Smad2/Smad2) was estimated. A representation of 2 analyses is shown (a). The quantitative analysis of the immunoblots is shown (b). The relative level of P-Smad2 in cells treated only with TGF-β was taken as 100 % of TGF-β-stimulated Smad2 phosphorylation. The data from three different analyses represents the mean ± SD. **Significantly higher than that in cells treated with TGF-β alone (P < 0.01)

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Journal of Biomedical Science

ISSN: 1423-0127